Multiplexed and Extraction-Free Amplification for Simplified SARS-CoV-2 RT-PCR Tests
نویسندگان
چکیده
منابع مشابه
Serology Testing for SARS-CoV-2: Benefits and Challenges
As COVID-19 was declared as a pandemic by the World Health Organization (WHO) in March 2020, it is an emerging need to discuss different aspects of this pandemic. In any pandemic, valid and rapid laboratory diagnostic tests are critically important for early diagnosis, which will increase the rate of successful treatment and more importantly prevent the spread of the disease.
متن کاملSARS-CoV-2 infection: its psychiatric manifestations
Background: The clinical presentation of SARS-CoV-2 infection was initially dominated by respiratory symptoms. However the clinical spectrum is wide and neuropsychiatric syndromes are also a source of medical concern. Our aims are to present an atypical clinical presentation of SARS-CoV-2 infection characterized by auditory hallucinations and unusual behavior and to emphasize the diversity of ...
متن کاملRapid SARS-CoV-2 RT-LAMP assay setup using gene construct design approach
Background and Objective: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still an ongoing challenge in health system worldwide. Molecular detection methods including reverse transcription loop-mediated isothermal amplification (RT-LAMP) can help cutting off the transmission chain of the virus. In this study, prompt setting up of the RT-LAMP assays were investigated using ge...
متن کاملمروری به بررسی ایمونولوژیکی SARS-Cov-2
زمینه و هدف بیماری کووید-19 یک بیماری عفونی بهشدت مسری میباشد که توسط یک کروناویروس جدید ایجاد میشود که میتواند از انسان به انسان از طریق تماس نزدیک منتقل شود. هدف از مطالعه حاضر بررسی اطلاعات جدید در زمینه ایمونولوژی کووید-19 میباشد. مواد و روشها در مطالعه مروری حاضر، از موتورهای جستجوی Google و Google Scholar و مقالات نمایه شده در پایگاههای علمی معتبر بینالمللی از جمله Pub Med، MedLi...
متن کاملDevelopment and comparison of the real-time amplification based methods--NASBA-Beacon, RT-PCR taqman and RT-PCR hybridization probe assays--for the qualitative detection of sars coronavirus.
The aim of this study was to develop a rapid, sensitive and robust procedure for the qualitative detection of SARS coronavirus RNA. Three unique detection formats were developed for real-time RNA amplification assays: a post amplification detection step with a virus-specific internal capture probe based on Taqman (RT-PCR TaqMan assay), hybridization probe (RT-PCR hybridization probe assay) and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2021
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.0c03918